biologia cellulare e molecolare karp pdf files

Presence of R8 was detected using SDS-PAGE and Western blotting. The highly efficient and useful Tovacco mosaic virus RNA-based overexpression (TRBO) vector was used to investigate expression of the R8 molecule in tobacco by agroinfection. This study explores whether a chimeric allergen R8, derived from the major allergen group 1 of house dust mites species (Dermatophagoides farinae and Dermatophagoides pteronyssinus), is expressed in tobacco. Using plants as a bioreactor for mass production of target proteins for vaccines is thought to show the most potential. Plants are widely accepted as a general platform for the large-scale production of recombinant proteins, which has been demonstrated by the successful expression of various exogenous proteins. Streptococcus pneumoniae, Tuberculosis Rabies, , Topical microbicides, Hepatitis A virus, Nerve Agents, , Hookworm, Cholera, ,, Tularemia Livestock/animal diseases, ,, , Venom anti-toxin Asthma/allergins, , Cancer treatment, ,, , Cocaine addiction Atherosclerosis Wound-healing factors, Abdelghani, 2015: 25984768] Human growth factors, a PMID-PubMed identifier from the National Center for Biotechnology Information (NCBI) online database. These results pave the way for the development of diagnostic allergy tests based on in planta produced allergens. In this paper we provide a proof of concept for the production of an immunologically active form of Der p 1 using a plant viral vector. The binding properties of in planta produced I-rDer p 1 versus the IgE of patients sera were comparable to those obtained on Der p 1 preparation immobilized on a microarray. Human sera from characterized allergic patients were used for IgE binding inhibition assays to test the immunological reactivity of I-rDer p 1 produced in N.

pteronyssinus and the PVX-mediated expression of its mature form (I-rDer p 1) in Nicotiana benthamiana plants. Here we report the isolation of the Der p 1 gene from an Italian strain of D. pteronyssinus, is found in high concentration in mites faecal pellets, which can became easily airborne and, when inhaled, can cause perennial rhinitis and bronchial asthma. Der p 1 protein, one of the main allergens of D. European (Dermatophagoides pteronyssinus) and American (Dermatophagoides farinae) house dust mite species are considered the most common causes of asthma and allergic symptoms worldwide.

Biologia cellulare e molecolare karp pdf files